Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / RNA-based Reprogramming of Human Primary…
JoVE Journal
Developmental Biology

This content is Open Access.

JoVE Journal Developmental Biology
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
 

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Article DOI: 10.3791/58687-v • 11:39 min • November 26th, 2018
November 26th, 2018 •
1,2,3, 1,2, 1,2,3, 1,2,3
1Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

Chapters

Summary
Automatic Translation

Here we describe a clinically relevant, high-efficiency, feeder-free method to reprogram human primary fibroblasts into induced pluripotent stem cells using modified mRNAs encoding reprogramming factors and mature microRNA-367/302 mimics. Also included are methods to assess reprogramming efficiency, expand clonal iPSC colonies, and confirm expression of the pluripotency marker TRA-1-60.

Tags

Keywords: RNA-based Reprogramming Induced Pluripotent Stem Cells IPSCs Primary Fibroblasts Transfection Efficiency PH Adjustment Laminin 521 Fibroblast Expansion Medium Plating Medium FGF B18R
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter