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在研究隐球菌-阿米巴相互作用中,显微技术和荧光读数的补充应用
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
DOI:

10:56 min

June 22, 2019

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Chapters

  • 00:00Title
  • 00:40Cultivation and Standardization of Fungal Cells
  • 01:54Cultivation and Standardization of Amoeba Cells
  • 02:34Fluorescence Staining of Cells to Study Phagocytosis
  • 04:50Usage of Transmission Electron Microscopy to Study Phagocytosis
  • 07:02Results
  • 09:31Conclusion

Summary

Automatic Translation

本文详细介绍了利用静止光图像和高分辨率透射电子显微镜图像进行制备隐球细胞和阿米巴共同培养的协议。此处说明定量数据如何补充此类定性信息。

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