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Journal / Biology / Isolation of Mouse Epidermal Keratinocytes and…
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Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture
 

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

Article DOI: 10.3791/58701-v • 06:16 min • August 10th, 2019
August 10th, 2019 •
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1Hormel Institute, University of Minnesota

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Summary
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The goal of this protocol is to isolate epidermal keratinocytes from the dorsal skin of adult mice for a variety of downstream applications such as molecular biology, biochemistry, fluorescence activated cell sorting, and primary in vitro uses (e.g., clonogenic keratinocytes).

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Keywords: Mouse Epidermal Keratinocytes Keratinocyte Stem Cells Skin Carcinogenesis Skin Tissue Epidermal Cell Harvesting Trypsin Epidermal Cell Culture Hair Cells Stratum Corneum
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