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Immunology and Infection

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JoVE Journal Immunology and Infection
High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
 

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

Article DOI: 10.3791/58753-v • 07:18 min • January 22nd, 2019
January 22nd, 2019 •
1, 1
1Department of Microbiology and Immunology, University of Melbourne

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Summary
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A high throughput protocol for functional assessment of HIV efficient reactivation and clearance of latent proviruses is described and applied by testing the impact of interventions on HIV transcription and splicing. Representative results of the effect of latency reversing agents on LTR-driven transcription and splicing are provided.

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Keywords: HIV Latency Reversing Agents Transcription Splicing In Vitro Assessment High Throughput Reporter Construct Transfection Cell Culture Flow Cytometry
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