Journal
/
Engineering
/
Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
JoVE Journal
Engineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Engineering
Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

DOI:
10.3791/58781-v

•

08:39 min

•

January 07, 2019

•

,
1Graduate College of Biomedical Sciences,Western University of Health Sciences

Chapters

  • 00:04Title
  • 00:31Growing Cells on Acoustically-Transparent Polyester Film
  • 02:34LIPUS Implementation and Oblique Acoustic Excitation
  • 03:29Driving the Transducer
  • 04:24Beam Alignment and Determination of Ultrasound Pulse Pressure and Intensity
  • 06:32Calcium-Sensitive/LIPUS Live-Cell Fluorescence Imaging
  • 07:33Results: LIPUS-induced Calcium Signals in Human Glioblastoma Cells A-172
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

Low-Intensity Pulsed Ultrasound Stimulation (LIPUS) is a modality for non-invasive mechanical stimulation of endogenous or engineered cells with high spatial and temporal resolution. This article describes how to implement LIPUS to an epi-fluorescence microscope and how to minimize acoustic impedance mismatch along the ultrasound path to prevent unwanted mechanical artefacts.

Tags

Keywords: MultiplexingFocused Ultrasound StimulationFluorescence MicroscopyMechanobiologyMechanical ForcesNoninvasiveCell CultureExtracellular MatrixPolyester FilmUV SterilizationTranslation StagesImmersion Transducer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58781?article_type=v

Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation
check_url/58781?article_type=v

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
check_url/58781?article_type=v

Fabrication of Gate-tunable Graphene Devices for Scanning Tunneling Microscopy Studies with Coulomb Impurities
check_url/58781?article_type=v

Ohmic Contact Fabrication Using a Focused-ion Beam Technique and Electrical Characterization for Layer Semiconductor Nanostructures
check_url/58781?article_type=v

In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography (CT) and Light Microscopy (LM) Correlated with Scanning Electron Microscopy (SEM)
check_url/58781?article_type=v

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
check_url/58781?article_type=v

Focused Ion Beam Fabrication of LiPON-based Solid-state Lithium-ion Nanobatteries for In Situ Testing
check_url/58781?article_type=v

Three-Dimensional Ultrasonic Needle Tip Tracking with a Fiber-Optic Ultrasound Receiver
check_url/58781?article_type=v

Controllable Nucleation of Cavitation from Plasmonic Gold Nanoparticles for Enhancing High Intensity Focused Ultrasound Applications
check_url/58781?article_type=v

Evaluating Targeting Accuracy in the Focal Plane for an Ultrasound-guided High-intensity Focused Ultrasound Phased-array System

Read Article