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荧光显微镜对聚焦超声的刺激
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JoVE Journal Engineering
Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
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荧光显微镜对聚焦超声的刺激

DOI:
10.3791/58781-v

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08:39 min

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January 07, 2019

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,
1Graduate College of Biomedical Sciences,Western University of Health Sciences

Chapters

  • 00:04Title
  • 00:31Growing Cells on Acoustically-Transparent Polyester Film
  • 02:34LIPUS Implementation and Oblique Acoustic Excitation
  • 03:29Driving the Transducer
  • 04:24Beam Alignment and Determination of Ultrasound Pulse Pressure and Intensity
  • 06:32Calcium-Sensitive/LIPUS Live-Cell Fluorescence Imaging
  • 07:33Results: LIPUS-induced Calcium Signals in Human Glioblastoma Cells A-172
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

低强度脉冲超声刺激 (lipus) 是一种非侵入性机械刺激的方式内源性或工程细胞具有较高的空间和时间分辨率。本文介绍了如何将 lipus 应用于荧光显微镜, 以及如何最大限度地减少超声路径上的声阻抗不匹配, 以防止不需要的机械文物。

Tags

Keywords: MultiplexingFocused Ultrasound StimulationFluorescence MicroscopyMechanobiologyMechanical ForcesNoninvasiveCell CultureExtracellular MatrixPolyester FilmUV SterilizationTranslation StagesImmersion Transducer

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