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Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions
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Cancer Research
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JoVE Journal Cancer Research
Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions

Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions

DOI:
10.3791/58825-v

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09:30 min

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January 07, 2019

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, , , ,
1Genetics and Bioengineering,Yeditepe University, 2National Heart, Lung, and Blood Institute (NHLBI), Sickle Cell Branch,National Institutes of Health (NIH)

Chapters

  • 00:04Title
  • 00:38Dental Pulp Stem Cell (DPSC) Isolation and Culture
  • 02:14DPSC Characterization and Differentiation
  • 04:01Conditioned Medium Cancer Cell Treatment
  • 05:09Cell Migration and Co-Culture Analyses
  • 07:23Results: Representative Effects of Tumor Cell:DPSC Interactions
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

We provide protocols for evaluation of mesenchymal stem cells isolated from dental pulp and prostate cancer cell interactions based on direct and indirect co-culture methods. Condition medium and trans-well membranes are suitable to analyze indirect paracrine activity. Seeding differentially stained cells together is an appropriate model for direct cell-cell interaction.

Tags

Keywords: Mesenchymal Stem CellsCancer CellsCo-cultureCell InteractionsStem Cell IsolationCell CultureCell MorphologySurface MarkersCell FixationCell Staining

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