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Journal / Cancer Research / Mesenchymal Stem Cell Isolation from Pulp Tissue…
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Cancer Research

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JoVE Journal Cancer Research
Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions
 

Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions

Article DOI: 10.3791/58825-v • 09:30 min • January 7th, 2019
January 7th, 2019 •
1, 2, 1, 1, 1
1Genetics and Bioengineering, Yeditepe University, 2National Heart, Lung, and Blood Institute (NHLBI), Sickle Cell Branch, National Institutes of Health (NIH)

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Summary
Automatic Translation

We provide protocols for evaluation of mesenchymal stem cells isolated from dental pulp and prostate cancer cell interactions based on direct and indirect co-culture methods. Condition medium and trans-well membranes are suitable to analyze indirect paracrine activity. Seeding differentially stained cells together is an appropriate model for direct cell-cell interaction.

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Keywords: Mesenchymal Stem Cells Pulp Tissue Cancer Cells Co-culture Cell Interactions Stem Cell Isolation Cell Culture Cell Morphology Surface Markers Cell Fixation Cell Staining
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