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Verifiserer nikotinsyre acetylcholin reseptor-funksjonen i musen hjernen skiver via Laser Flash Photolysis Photoactivatable nikotin
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

Verifiserer nikotinsyre acetylcholin reseptor-funksjonen i musen hjernen skiver via Laser Flash Photolysis Photoactivatable nikotin

DOI:
10.3791/58873-v

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10:48 min

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January 25, 2019

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, , , ,
1Department of Pharmacology,Northwestern University Feinberg School of Medicine, 2Department of Physiology,Northwestern University Feinberg School of Medicine, 3Janelia Research Campus,Howard Hughes Medical Institute

Chapters

  • 00:04Title
  • 00:39Preparation of Photoactivatable Nicotine (PA-Nic)
  • 03:23Imaging Neurons with 2-Photon Laser Scanning Microscopy
  • 05:28Calibration of Uncaging Laser Galvanometers and Pairing of PA-Nic Laser Flash Photolysis with Electrophysiological Recordings
  • 07:47Results: Representative 2-Photon Laser Scanning Microscopy Imaging and PA-Nic Laser Flash Photolysis Responses
  • 09:47Conclusion

Summary

Automatic Translation

Automatic Translation

Denne artikkelen presenterer en metode for å studere nikotinsyre acetylkolin reseptorer (nAChRs) i musen hjernen skiver av nikotin uncaging. Når kombinert med samtidige oppdateringen klemme opptak og 2-fotonet laserskanning mikroskopi, forbinder nikotin uncaging nikotinsyre reseptoren funksjonen med cellular morfologi, gir en dypere forståelse av cholinergic nevrobiologi.

Tags

Laser Flash PhotolysisPhotoactivatable NicotineNicotinic Acetylcholine ReceptorsMouse Brain SlicesMulti-photon Laser-scanning MicroscopySpatiotemporal ControlCholinergic Synaptic TransmissionVolume TransmissionPatch ClampPressure EjectionBath ApplicationPerfusion System

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