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Neuroscience
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自体影像学作为一种简单而有力的药理靶标可视化和表征方法
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
Please note that all translations are automatically generated.
Click here for the English version.
自体影像学作为一种简单而有力的药理靶标可视化和表征方法
DOI:
10.3791/58879-v
•
10:16 min
•
March 12, 2019
•
Nane Griem-Krey
,
Anders Bue Klein
,
Matthias Herth
2,3
,
Petrine Wellendorph
1
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences
,
University of Copenhagen
,
2
Neurobiology Research Unit and CIMBI
,
Copenhagen University Hospital
,
3
Department of Clinical Physiology, Nuclear Medicine & PET
,
Copenhagen University Hospital
Chapters
00:04
Title
00:46
Tissue Preparation by Cryosectioning
02:13
In vitro Autoradiography
04:44
Exposure to Phosphor Imaging Plates and Scanning
06:04
Densitometric Analysis of Digital Image
07:34
Results: Visualization and Characterization of Pharmacological Targets Using Radiography
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
自体影像学方法通常用于研究放射线与组织切片的结合, 以确定定性或定量药理学。
Tags
Autoradiography
Protein Binding
Pharmacological Targets
Cryostat Sectioning
Radioligand Labeling
Non-specific Binding
Spatial Resolution
Digital Imaging
Pharmacological Analysis
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