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体外检测评估神仙体头三个月滋养细胞细胞系的迁移、入侵和增殖

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Developmental Biology

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JoVE Journal Developmental Biology

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

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体外检测评估神仙体头三个月滋养细胞细胞系的迁移、入侵和增殖

DOI:

10.3791/58942-v

•

09:57 min

•

March 05, 2019

•

Edwina P. Kisanga, Zhonghua Tang, Seth Guller, Shannon Whirledge

¹Department of Obstetrics, Gynecology and Reproductive Sciences,Yale School of Medicine

Chapters

00:04Title
00:24Scratch Assay
02:05Invasion Assay
06:26Proliferation Assay
08:06Results: Investigating the Role of Glucocorticoids in Trophoblast Cell Migration
09:23Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个高度可访问的协议, 用于评估细胞在人类滋养细胞中的运动使用三个体外检测: 划痕试验, 转井入侵试验, 细胞增殖试验。

Tags

Trophoblast Invasion Migration Proliferation In Vitro Assay Scratch Assay Invasion Assay Extracellular Matrix Cell Culture Obstetrical Outcomes

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