JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Please note that all translations are automatically generated.
Click here for the English version.
放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析
DOI:
10.3791/58952-v
•
08:59 min
•
February 12, 2019
•
Nicholas McCaul
2
,
Hui Ying Yeoh
,
Guus van Zadelhoff
,
Naomi Lodder
,
Bertrand Kleizen
,
Ineke Braakman
1
Cellular Protein Chemistry
,
Utrecht University
,
2
Program in Cellular and Molecular Medicine
,
Boston Children’s Hospital
Chapters
00:04
Title
01:05
Pulse Chase for Adherent Cells
04:07
Pulse Chase for Suspension Cells
05:03
Immunoprecipitation
06:16
Analysis of the Radioactive Pulse Chase Results
07:58
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里, 我们描述了一种一般脉冲追逐方法的协议, 该方法允许在活细胞中对蛋白质的折叠、迁移和降解进行动力学分析。
Tags
Protein Folding
Protein Transport
Protein Degradation
Radioactive Pulse Chase
35S-labeled Methionine And Cysteine
Protein Biosynthesis
Co- And Post-translational Modifications
Disulfide Bond Formation
Acetylation
Cell Culture
Transfection
Starvation Medium
Pulse Solution
Chase Medium
Stop Buffer
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/58952?article_type=v
方法确定HCN1和TRIP8b之间蛋白 - 蛋白相互作用的小分子抑制剂
check_url/58952?article_type=v
碳水化合物转运基质结合蛋白 SP0092 的生化和结构表征
check_url/58952?article_type=v
原位
近接结扎法检测蛋白异构体的 Heterodimerization
check_url/58952?article_type=v
用石英谐振器研究磷脂结合蛋白附件 a2 与固体支持的脂质层结合动力学的耗散微重法
check_url/58952?article_type=v
蓝色本机凝胶电泳法分析囊体膜蛋白配合物
check_url/58952?article_type=v
生理活性类囊体的分离及其在能量依赖性蛋白转运检测中的应用
check_url/58952?article_type=v
荧光显微镜竞争滴定法测定转录机-dna 结合感的高灵敏度
check_url/58952?article_type=v
培养细胞功能化纳米化对跨 golgi 网络的内分泌吸收和逆行迁移分析
check_url/58952?article_type=v
使用开源 MALDI TOF-MS IDBac 管道分析微生物蛋白和专用代谢物数据
check_url/58952?article_type=v
通过组合单域抗体库的分步噬菌体选择创建高度特异性化学诱导蛋白分解系统
Read Article