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An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay
 

An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay

Article DOI: 10.3791/58966-v • 09:47 min • February 1st, 2019
February 1st, 2019 •
1, 1
1College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University

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Summary
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Here, we present a protocol for a novel gap junction intercellular communication assay designed for the high-throughput screening of gap junction-modulating chemicals for drug discovery and toxicological assessment.

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Keywords: Iodide-YFP Gap Junction Intercellular Communication High-throughput Screening Assay LN215 Cells Lentivirus Puromycin Cell Culture
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