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通过纵向荧光显微镜监测新生儿生存
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy
DOI:

07:02 min

January 19, 2019

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Chapters

  • 00:04Title
  • 01:06Transfection of Rat Cortical Neurons
  • 02:22Imaging
  • 03:09Image Processing and Scoring Cell Death
  • 04:35Performing Cox Proportional Hazards Analysis and Visualizing Results
  • 05:19Results: Assessing Neuronal Survival on a Single-cell Basis
  • 06:16Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 在单细胞的基础上监测生存情况, 并确定显著预测细胞死亡的变量。

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