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Journal
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Neuroscience
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通过纵向荧光显微镜监测新生儿生存
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
通过纵向荧光显微镜监测新生儿生存
DOI:
10.3791/59036-v
•
07:02 min
•
January 19, 2019
•
Kaitlin Weskamp*
1,2
,
Nathaniel Safren*
1
,
Roberto Miguez
,
Sami Barmada
2
1
Department of Neurology
,
University of Michigan School of Medicine
,
2
Neuroscience Graduate Program
,
University of Michigan School of Medicine
Chapters
00:04
Title
01:06
Transfection of Rat Cortical Neurons
02:22
Imaging
03:09
Image Processing and Scoring Cell Death
04:35
Performing Cox Proportional Hazards Analysis and Visualizing Results
05:19
Results: Assessing Neuronal Survival on a Single-cell Basis
06:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个协议, 在单细胞的基础上监测生存情况, 并确定显著预测细胞死亡的变量。
Tags
Keywords: Neuronal Survival
Longitudinal Fluorescence Microscopy
Single-cell Monitoring
Cytotoxicity Assays
Neurodegenerative Diseases
Cell Death
Transfection Efficiency
Multichannel Pipet
Cortical Neurons
NBKY Solution
Conditioned Media
NBC Solution
Fluorescent Microscope
Fiduciary Mark
X And Y Coordinates
Fluorescent Label
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