Journal
/
/
墨西哥鱼基因功能的操纵
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Manipulation of Gene Function in Mexican Cavefish
DOI:

07:01 min

April 22, 2019

, , , , ,

Chapters

  • 00:04Title
  • 00:47Tool Preparation and Single-Cell Stage Egg Collection
  • 01:34Pico-Injection
  • 03:36Screening of Morpholino-Injected Individuals, CRISPR Mutagenesis, and Tol2 Transmission
  • 04:35Results: Representative Post-Pico-Injection Functional and Phenotypical Analyses
  • 06:27Conclusion

Summary

Automatic Translation

我们描述了在进化模型系统中操纵基因的方法. 描述了三种不同的技术: Tol2 介导的转基因、使用 CRISPR/Cas9 对基因组进行有针对性的操纵以及使用吗啡酮敲除表达。这些工具应有助于直接研究表面和洞穴居住形式之间变异背后的基因。

Related Videos

Read Article