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Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
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Chapters
- 00:04Title
- 01:05Preparation of A549 Cells and JUNV (Junn virus) Infection
- 02:09Fixation and Immunostaining
- 05:10Results: Distribution and Colocalization Between the Retinoic Acid Like Receptors RLRs (RIG-I and MDA-5) and dsRNA in JUNV-infected Cells
- 05:52Conclusion
Double-stranded RNA produced during RNA virus replication can be recognized by pattern recognition receptors to induce an innate immune response. For negative-sense RNA viruses, the interaction between the low-level dsRNA and PRRs remains unclear. We have developed a confocal microscopy method to visualize arenavirus dsRNA and PRR in individual cells.
