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Mimicking and Manipulating Pancreatic Acinar-to-Ductal Metaplasia in 3-dimensional Cell Culture
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Cancer Research
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JoVE Journal Cancer Research
Mimicking and Manipulating Pancreatic Acinar-to-Ductal Metaplasia in 3-dimensional Cell Culture

Mimicking and Manipulating Pancreatic Acinar-to-Ductal Metaplasia in 3-dimensional Cell Culture

DOI:
10.3791/59096-v

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08:16 min

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February 11, 2019

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,
1Department of Cancer Biology,Mayo Clinic Florida

Chapters

  • 00:04Title
  • 00:30Acinar Cell Isolation
  • 05:11Viral Infection
  • 05:50Embedment of Cells in Collagen and Basement Membrane Matrix
  • 07:12Results: Primary Acinar Cells Plated within Collagen or Basement Membrane Matrix after Adenoviral Infection and Stimulation with TGF–α
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

Primary acinar cell isolation, protein expression or activity modulation, culture, and down-stream applications are abundantly useful in the ex vivo study of acinar-to-ductal metaplasia (ADM), an early event in the development of pancreatic cancer.

Tags

Pancreatic Acinar-to-ductal Metaplasia3D Cell CultureReal-time VisualizationCell Signaling StudiesPancreas DissectionCollagenase Digestion

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