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在异种性鼠模型中的移植-无源疾病的诱导和评分,以及使用数字PCR对小鼠组织中人类T细胞的定量
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR
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在异种性鼠模型中的移植-无源疾病的诱导和评分,以及使用数字PCR对小鼠组织中人类T细胞的定量

DOI:
10.3791/59107-v

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06:06 min

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May 23, 2019

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1Department of Microbiology, Molecular Genetics, and Immunology,University of Kansas Medical Center

Chapters

  • 00:04Title
  • 00:40Non-Obese Diabetic (NOD) Scid Gamma (NSG) Mouse Irradiation and Peripheral Blood Mononuclear Cell (PBMC) Injection
  • 02:22Retro-Orbital Injection
  • 03:24Clinical Graft Versus Host Disease (GVHD) Scoring
  • 03:58Genomic DNA Isolation and Human T Cell Quantification
  • 04:59Results: Representative GVHD Disease Progression and Digital PCR Human T Cell Detection
  • 05:37Conclusion

Summary

Automatic Translation

Automatic Translation

在这里,我们提出了一个协议,以诱导和评分疾病在异种移植-宿主疾病(xenoGVHD)模型。xenoGVHD为研究人类T细胞的免疫抑制提供了一个体内模型。此外,我们介绍如何使用数字PCR检测组织中的人类T细胞,作为量化免疫抑制的工具。

Tags

Keywords: Xenograft-versus-host DiseaseXenoGVHDNSG MiceIrradiationPeripheral Blood Mononuclear CellsPBMCsDensity Gradient CentrifugationRetro-orbital InjectionHuman T CellsImmunosuppressive Therapies

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