Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Environment

A subscription to JoVE is required to view this content. Sign in or start your free trial.

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
 

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Article DOI: 10.3791/59119-v 08:31 min March 29th, 2019
March 29th, 2019

Chapters

Summary

Here, we present two protocols to transform potato plants. The Agrobacterium tumefaciens transformation leads to a complete transgenic plant while the Agrobacterium rhizogenes produces transgenic hairy roots in a wild type shoot that can be self-propagated. We then detect promoter activity by GUS staining in the transformed roots.

Tags

Keywords: Agrobacterium Tumefaciens Agrobacterium Rhizogenes Potato Transformation Gene Function Root Transformation GUS Staining Hairy Roots Composite Plant
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter