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细胞调理介质细胞外囊泡的流式细胞学分析
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JoVE Journal Biology
Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
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细胞调理介质细胞外囊泡的流式细胞学分析

DOI:
10.3791/59128-v

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08:32 min

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February 12, 2019

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, , ,
1Cellular and Molecular Cardiology Laboratory,Cardiocentro Ticino Foundation, Switzerland, 2Molecular Cardiology Institute, Dept. of Cardiology,University of Zürich, 3Faculty of Biomedical Science,Università Svizzera Italiana, Switzerland

Chapters

  • 00:04Title
  • 01:05Conditioned Medium Collection and Processing
  • 02:45Nanoparticle Tracking Analysis (NTA) Quantification
  • 03:27Sample Preparation
  • 04:17Sample Acquisition and Analysis
  • 06:32Results: Representative Flow Cytometric Analyses of Extracellular Vesicles from Cell-conditioned Medium
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种可重复的方法, 该方法设计用于细胞培养上清液, 以检测小细胞外囊泡 (ev) 上的表面表位。它利用特定的 ev 免疫沉淀使用珠子与抗体结合, 识别表面抗原 cd9, cd63 和 cd81。该方法对下游流式细胞仪分析进行了优化。

Tags

Flow CytometryExtracellular VesiclesCell-conditioned MediaBiomarkersTherapeutic ToolsDiagnosticParticle CharacterizationSurface MarkersCardiac Progenitor CellsExosome ProductionUltracentrifugationNanoparticle Tracking Analysis

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