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从环境样品中筛选高通量的安德森菌: 植物组织、散装土壤和根际土壤
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Environment
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JoVE Journal Environment
High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
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从环境样品中筛选高通量的安德森菌: 植物组织、散装土壤和根际土壤

DOI:
10.3791/59137-v

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12:36 min

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February 09, 2019

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, , , ,
1Department of Crop and Soil Sciences,Washington State University, 2Department of Plant Pathology,Washington State University

Chapters

  • 00:04Title
  • 01:01Preparation of Chrome Azurol S (CAS)-Fe-Agar Medium
  • 03:35Pyoverdine/EDTA Standard Preparation
  • 05:04Collection of Environmental Samples: Soil and Plant Tissues
  • 06:38Preparation of Siderophore Enrichment Cultures and CAS-Fe Siderophore Production Assay
  • 08:11CAS-Fe Agar Assays for Detection of Siderophore Production in Environmental Samples
  • 09:34Results: Pyoverdine Equivalents of Siderophore Enrichment Cultures
  • 11:28Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一个快速筛选环境样品的协议, 以寻找有助于在陆地系统中实现微量营养素生物利用度和周转的环境样本。

Tags

Siderophore ScreeningHigh-throughputEnvironmental SamplesBulk SoilsRhizosphere SoilsIron CyclingMicrobial CommunityAgriculture ProductivityFarm Management PracticesSoil HealthCAS AssayHDTMACAS SolutionPIPES Buffer SolutionAgarose

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