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Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

DOI:
10.3791/59167-v

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13:20 min

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March 07, 2019

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1Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences,University of Central Florida

Chapters

  • 00:04Title
  • 01:22Reagent and Cell Preparation for ROS Measurement
  • 05:02Assay Preparation
  • 05:53Performing the Assay
  • 11:28Results: Flow Cytometric Measurement of ROS Production in Macrophages in Response to FcγR Cross-linking
  • 13:00Conclusion

Summary

Automatic Translation

Automatic Translation

This study demonstrates the use of flow cytometry to detect reactive oxygen species (ROS) production resulting from activation of the FcγR. This method can be used to assess changes in the antimicrobial and redox signaling function of phagocytes in response to immune complexes, opsonized microorganisms, or direct FcγR cross-linking.

Tags

Keywords: Flow CytometryROS ProductionMacrophagesFc-gamma Receptor CrosslinkingOxidative StressSuperoxideChronic Granulomatous DiseaseAutoimmune DiseasesNeurodegenerative Diseases

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