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Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
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Chapters
- 00:05Title
- 00:49PM10 Sampling
- 02:54Bet v 1 Staining
- 06:04Flow Cytometric Analysis
- 08:29Results: High Pollen Season Results in Greater Number of Particles with Bound Bet v 1 Allergen
- 09:18Conclusion
Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.
