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Immunology and Infection

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JoVE Journal Immunology and Infection
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
 

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Article DOI: 10.3791/52771-v • 11:07 min • April 29th, 2015
April 29th, 2015 •
1,2, 1,2,3
1Department of Pharmaceutical Sciences, University of Michigan, 2Biointerfaces Institute, University of Michigan, 3Department of Biomedical Engineering, University of Michigan

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Summary
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We describe whole-animal imaging and flow cytometry-based techniques for monitoring expansion of antigen-specific CD8+ T cells in response to immunization with nanoparticles in a murine model of vaccination.

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Keywords: Nanoparticle Vaccine CD8+ T Cell Whole-animal Imaging Flow Cytometry Antigen-specific T Cell Response Interbilayer-crosslinked Multilamellar Vesicles (ICMVs) Bioluminescence Imaging Tetramer Staining
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