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Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis

Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis

DOI:
10.3791/61559-v

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06:07 min

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June 27, 2020

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, ,
1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine,University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital,Central South University

Chapters

  • 00:04Introduction
  • 00:56Intravascular Labeling of CD8+ T Cells
  • 02:25Enzymatic Digestion of the Kidney
  • 03:21Density Gradient Centrifugation to Enrich Lymphocytes from the Digested Kidney
  • 04:46Results: Flow Cytometry Analysis of Kidney Lymphocytes
  • 05:40Conclusion

Summary

Automatic Translation

Automatic Translation

Following viral infection, kidney harbors a relatively large number of CD8+ T cells and offers an opportunity to study non-mucosal TRM cells. Here, we describe a protocol to isolate mouse kidney lymphocytes for flow cytometry analysis.

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Keywords: Mouse KidneyCD8+ T CellsFlow CytometryBiotin Anti-CD8 AntibodyHomogenization

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