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Breath Collection from Children for Disease Biomarker Discovery
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JoVE Journal Chemistry
Breath Collection from Children for Disease Biomarker Discovery

Breath Collection from Children for Disease Biomarker Discovery

DOI:
10.3791/59217-v

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06:09 min

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February 14, 2019

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, , ,
1Department of Pediatrics,Washington University School of Medicine, 2Cell Biology & Physiology,Washington University School of Medicine, 3Division of Gastroenterology, Hepatology and Nutrition,Washington University School of Medicine, 4Department of Molecular Microbiology,Washington University School of Medicine

Chapters

  • 00:04Title
  • 00:54Breath Sample Equipment Assembly
  • 01:42Breath Collection
  • 02:46Ambient Air Collection
  • 03:35Thermal Desorption Tube Breath Transfer
  • 04:33Results: Representative Exhaled Breath Volatile Assessment
  • 05:39Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a simple method for the acquisition of breath samples from children. Briefly, samples of mixed air are pre-concentrated in sorbent tubes prior to gas chromatography-mass spectrometry analysis. Breath biomarkers of infectious and non-infectious diseases can be identified using this breath collection method.

Tags

Keywords: Breath CollectionChildrenDisease BiomarkersNoninvasiveVisual InstructionBreath SamplerBag ValvePatient IDBreath ExhalationLiter Of Breath

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