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Journal
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Chemistry
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儿童疾病生物标志物发现的呼吸收集
JoVE Journal
Chemistry
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JoVE Journal
Chemistry
Breath Collection from Children for Disease Biomarker Discovery
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儿童疾病生物标志物发现的呼吸收集
DOI:
10.3791/59217-v
•
06:09 min
•
February 14, 2019
•
Amalia Z. Berna
,
Brian DeBosch
2
,
Janis Stoll
,
Audrey R. Odom John
4
1
Department of Pediatrics
,
Washington University School of Medicine
,
2
Cell Biology & Physiology
,
Washington University School of Medicine
,
3
Division of Gastroenterology, Hepatology and Nutrition
,
Washington University School of Medicine
,
4
Department of Molecular Microbiology
,
Washington University School of Medicine
Chapters
00:04
Title
00:54
Breath Sample Equipment Assembly
01:42
Breath Collection
02:46
Ambient Air Collection
03:35
Thermal Desorption Tube Breath Transfer
04:33
Results: Representative Exhaled Breath Volatile Assessment
05:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了一种从儿童身上采集呼吸样本的简单方法。简单地说, 混合空气样品在气相色谱-质谱分析之前, 先在吸附剂管中进行预浓缩。使用这种呼吸收集方法可以确定传染病和非传染性疾病的呼吸生物标志物。
Tags
Keywords: Breath Collection
Children
Disease Biomarkers
Noninvasive
Visual Instruction
Breath Sampler
Bag Valve
Patient ID
Breath Exhalation
Liter Of Breath
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