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在 Vivo 三维双光子显微镜中,利用玻璃微移器研究本地 ATP 喷射的血管反应

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Neuroscience

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JoVE Journal Neuroscience

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

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在 Vivo 三维双光子显微镜中,利用玻璃微移器研究本地 ATP 喷射的血管反应

DOI:

10.3791/59286-v

•

10:54 min

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June 07, 2019

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Changsi Cai, Stefan A. Zambach, Jonas C. Fordsmann, Micael Lønstrup, Kirsten J. Thomsen, Aske G. K. Jensen, Martin Lauritzen²

¹Department of Neuroscience, Faculty of Medicine and Health Science,University of Copenhagen, ²Department of Clinical Neurophysiology,Rigshospitalet

Chapters

00:04Title
00:57Surgical Procedure: Tracheotomy
01:55Surgical Procedure: Catheter Insertion
02:59Surgical Procedure: Craniotomy
05:29Two-photon Imaging
06:32Insertion of the Glass Micro-pipette
09:11Results: ATP Puffing by Micro-pipette Induces Vessel Dilation, followed by Constriction
10:30Conclusion

Summary

Automatic Translation

我们使用玻璃微移液器和快速的双光子超堆成像方法提供优化的局部弹出程序,从而能够精确测量毛细管直径变化,并在三维中调查其调节。

Tags

Keywords: Three-dimensional Two-photon Microscopy Conducted Vascular Responses ATP Ejection Glass Micro-pipette Cerebral Capillaries Cerebral Blood Flow Regulation Astrocytic Calcium Responses

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