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Growth and Characterization of Irradiated Organoids from Mammary Glands
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Bioengineering
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JoVE Journal Bioengineering
Growth and Characterization of Irradiated Organoids from Mammary Glands

Growth and Characterization of Irradiated Organoids from Mammary Glands

DOI:
10.3791/59293-v

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09:49 min

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May 03, 2019

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, , ,
1Department of Chemical and Biomolecular Engineering,Vanderbilt University, 2Department of Biomedical Engineering,Vanderbilt University, 3Department of Radiation Oncology,Vanderbilt University Medical Center

Chapters

  • 00:04Title
  • 00:55Preparation of Mice and Cell Acquisition
  • 04:19Plating Organoids and Co-culturing with Macrophages
  • 05:27Immunofluorescence Staining of Organoids
  • 07:08Results: Irradiated Epithelial Mammary Organoids were Cultured on Low-Adhesion Plates, Stained for Various Markers, and Co-Cultured with Immune Cells
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

Organoids developed from mouse mammary glands were irradiated and characterized to assess epithelial traits and interactions with immune cells. Irradiated organoids can be used to better evaluate cell-cell interactions that may lead to tumor cell recruitment in irradiated normal tissue.

Tags

Keywords: Mammary GlandsOrganoidsIonizing RadiationTumor Cell RecruitmentCancer RecurrenceTriple-negative Breast CancerConfocal MicroscopyCollagenaseEpithelial CellsStromal CellsRed Blood CellsBSA Solution

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