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从茶中提取的新建细胞悬浮培养基中六种系统杀虫剂的代谢研究(山茶西宁西斯L.叶

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Biochemistry
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JoVE Journal Biochemistry

Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves

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从茶中提取的新建细胞悬浮培养基中六种系统杀虫剂的代谢研究(山茶西宁西斯L.叶

DOI:

10.3791/59312-v

•

10:35 min

•

June 15, 2019

•

Weiting Jiao*^1,2, Guoqin Ge*¹, Rimao Hua, Jun Sun, Yeyun Li, Ruyan Hou

¹State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology,Anhui Province Key Lab of Analysis and Detection for Food Safety, ²School of Resource & Environment, Anhui Agricultural University,Key Laboratory of Agri-food Safety of Anhui Province

Chapters

00:00Title
01:46Tea Callus Culture
02:46Tea Cell Suspension Culture
03:43TTC Assay of Cell Viability
04:25Treatment and Sampling of Tea Cell Suspension Cultures with Insecticides
06:24Sample Preparation of Insecticides in Intact Tea Plant
06:56Instrument Analysis
07:54Results
10:02Conclusion

Summary

Automatic Translation

这项工作提出了建立从茶(山茶)叶衍生的细胞悬浮培养物的协议,可用于研究整个植物可以采用的外部化合物的代谢,如杀虫剂。

Tags

Keywords: Tea Cell Suspension Culture Systemic Insecticides Metabolism Pesticide Degradation Tea Leaves Explant Callus Liquid Cell Suspension Plant Metabolism

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