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Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers
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Biochemistry
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JoVE Journal Biochemistry
Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

DOI:
10.3791/59331-v

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10:41 min

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June 24, 2019

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, , , , ,
1Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences,Beijing Institute of Technology, 2UCLA Institute for Technology Advancement (Suzhou)

Chapters

  • 00:04Title
  • 01:02Construction of the Plasmids Expressing the Rare-codon-rich Marker Genes
  • 01:50Optimizing the Selection Conditions
  • 03:23Optimizing the Screening Conditions
  • 04:23Identification of the Amino Acid Overproducers
  • 07:51Results: Identifying Amino Acid Overproducers
  • 09:43Conclusion

Summary

Automatic Translation

Automatic Translation

This study presents an alternative strategy to the conventional toxic analog-based method in identifying amino acid overproducers by using rare-codon-rich markers to achieve accuracy, sensitivity, and high-throughput simultaneously.

Tags

Keywords: Amino Acid OverproducersRare-codon-rich MarkersAnalog-based ApproachHigh ThroughputTranslation Of Rare CodonsMolecular Experimental OperationsSelection StringenciesVisual DemonstrationSelection And Screening SystemKanRRare Codon CTAWild-type Selection Marker GeneRare-codon-rich DerivativeOD600L-leucine

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