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Chapters
- 00:00Title
- 01:37Light Cycle Manipulation
- 02:25Adjusted Photoperiod and Priming Fish for Gamete Collection
- 04:09Female Gamete Collection
- 05:39Male Gamete Collection
- 07:13In-vitro Fertilization
- 08:22Results
- 10:17Conclusion
In vitro fertilization is a commonly used technique with a variety of model organisms to maintain lab populations and produce synchronized embryos for downstream applications. Here, we present a protocol that implements this technique for different populations of the Mexican tetra fish, Astyanax mexicanus.
