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二维和三维 i 型胶原蛋白基质角质形成细胞增殖的评价
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
DOI:

08:19 min

April 22, 2019

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Chapters

  • 00:04Title
  • 01:12Preparation of Keratinocyte Culture Medium and the Fibril Form of Type I Collagen
  • 02:53Preparation of the Non-fibrous Form of Type I Collagen
  • 03:50Culture of FEPE1L-8 Cells
  • 05:20Estimation of the Number of Viable Cells
  • 06:20Results: Evaluation of Keratinocyte Proliferation
  • 07:01Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 用于利用 I 型胶原蛋白制备两种不同形式的培养基板。根据胶原蛋白的处理方式, 胶原蛋白分子要么保持二维, 非纤维状的形式, 要么重新组装成三维的, 纤维形式。I 型胶原蛋白上的细胞增殖受到纤维形成的极大影响。

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