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Evaluering af keratinocytter spredning på to - og tre - dimensionelle Type I kollagen substrater
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates

Evaluering af keratinocytter spredning på to - og tre - dimensionelle Type I kollagen substrater

DOI:
10.3791/59339-v

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08:19 min

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April 22, 2019

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, , ,
1Nippi Research Institute of Biomatrix, 2Department of Anatomy and Cell Biology,Osaka Medical College

Chapters

  • 00:04Title
  • 01:12Preparation of Keratinocyte Culture Medium and the Fibril Form of Type I Collagen
  • 02:53Preparation of the Non-fibrous Form of Type I Collagen
  • 03:50Culture of FEPE1L-8 Cells
  • 05:20Estimation of the Number of Viable Cells
  • 06:20Results: Evaluation of Keratinocyte Proliferation
  • 07:01Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer her, en protokol for forberedelse af to forskellige former for kultur substrater udnytte type I-kollagen. Afhængigt af hvordan håndteres kollagen, kollagen molekyler enten opretholde todimensional, ikke-fiberholdigt form eller samle ind i tre-dimensionelle, fibril form. Type I-celledelingen på kollagen påvirkes kraftigt af fibril formation.

Tags

Keywords: CollagenType I Collagen2D Culture3D CultureCell CultureKeratinocytesProliferationSubstratePBSHydrochloric AcidCell BehaviorBiological EnvironmentCulture MediumGelationIncubation

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