Journal
/
Cancer Research
/
An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

DOI:
10.3791/59368-v

•

00:06 min

•

September 12, 2019

•

, , , , , ,
1Department of Surgery,University of Missouri-Columbia, 2Ellis Fischel Cancer Center,University of Missouri-Columbia, 3Molecular Microbiology and Immunology,University of Missouri-Columbia

Chapters

  • 00:04Title
  • 00:50Liver Fibrosis and Cirrhosis Induction
  • 01:19T Antigen (T Ag)-Transgenic Hepatocyte Isolation
  • 03:48MTD2 Hepatocyte Inoculation
  • 05:22Results: Representative Progress MTD2 Tumor Development
  • 05:58Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe the development of a clinically relevant murine model of liver cancer recapitulating the typical immune features of hepatocellular cancer (HCC).

Tags

Hepatocellular CancerOrthotopic Mouse ModelHepatic InflammationFibrosisTumor ImmunologyTumor MicroenvironmentLiver CancerTumor PerfusionCollagenase DigestionTumor Implantation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59368?article_type=v

Fluorescent Orthotopic Mouse Model of Pancreatic Cancer
check_url/59368?article_type=v

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
check_url/59368?article_type=v

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry
check_url/59368?article_type=v

Murine Model of Metastatic Liver Tumors in the Setting of Ischemia Reperfusion Injury
check_url/59368?article_type=v

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
check_url/59368?article_type=v

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma
check_url/59368?article_type=v

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity
check_url/59368?article_type=v

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
check_url/59368?article_type=v

An Orthotopic Resectional Mouse Model of Pancreatic Cancer
check_url/59368?article_type=v

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

Read Article