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Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species
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Developmental Biology
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JoVE Journal Developmental Biology
Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species

DOI:
10.3791/59369-v

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06:46 min

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March 29, 2019

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,
1Department of Botany and Plant Pathology,Purdue University, 2Purdue Center for Plant Biology,Purdue University

Chapters

  • 00:04Title
  • 00:36Plant Growth and Dissection of the Shoot Apex
  • 01:43Staining and Image Collection
  • 03:38Image Processing
  • 04:43Results: Live Imaging and Analyzing of the Inflorescence Shoot Apical Meristem of Arabidopsis and the Vegetative Shoot Apical Meristem of Tomato and Soybean
  • 06:19Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents how to live image and analyze the shoot apical meristems from different plant species using laser scanning confocal microscopy.

Tags

Confocal Live ImagingShoot Apical MeristemPlant SpeciesArabidopsisTomatoSoybeanPropidium Iodide StainingDissectionSample PreparationConfocal Microscopy

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