Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / A Simple Chamber for Long-term Confocal Imaging…
JoVE Journal
Developmental Biology

This content is Open Access.

JoVE Journal Developmental Biology
A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
 

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Article DOI: 10.3791/55331-v • 07:59 min • May 17th, 2017
May 17th, 2017 •
1, 1
1Department of Plant Sciences, University of Oxford

Chapters

Summary
Automatic Translation

Presented here is a simple technique for high-resolution confocal time-lapse imaging of root and hypocotyl development for up to 3 days using high numerical-aperture objectives and perfluorodecalin as an immersion medium.

Tags

Keywords: Confocal Microscopy Long-term Imaging Plant Development Root Hypocotyl Organogenesis Perfluorodecalin PDMS Agar Chamber Design
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter