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Immunology and Infection
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JoVE Journal Immunology and Infection
Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
 

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

Article DOI: 10.3791/59386-v • 07:30 min • June 15th, 2019
June 15th, 2019 •
*1,2, *1,2, *2,3, 1, 3, 2
1West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 2Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, 3State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University
* These authors contributed equally

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Summary
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This protocol describes techniques to measure infectious outcomes underlying secondary hospital-acquired infections in the immunosuppressive condition, first by establishing cecal ligation/puncture mice then challenging them with intranasal infection to create a clinically relevant model of immunosuppression sepsis.

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Keywords: Cecal Ligation Cecal Puncture Sepsis Immunosuppression Mouse Model Intranasal Infection Staphylococcus Aureus Pneumonia
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