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Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
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JoVE Journal Chemistry
Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

DOI:
10.3791/59411-v

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08:54 min

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June 05, 2019

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1Department of Chemistry and Biochemistry,Ohio University

Chapters

  • 00:04Title
  • 00:33Glass and Nanoparticle Sample Preparation
  • 02:03DIC Imaging Preparation
  • 03:22DIC Nanoparticle Sample Imaging
  • 04:41Data Analysis using ImageJ
  • 06:39Results: Spectroscopy of Plasmonic Nanoparticles
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to detail a proven approach for the preparation of plasmonic nanoparticle samples and for performing single particle spectroscopy on them with differential interference contrast (DIC) microscopy.

Tags

Plasmonic NanoparticlesTransmission-based Nomarski-type Differential Interference Contrast MicroscopyDIC MicroscopyDark FieldLateral ResolutionDepth Of FieldGold NanoparticlesCentrifugationResuspensionDropcastingKohler IlluminationHigh MagnificationDe Senarmont Design

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