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Journal / Biochemistry / Forbedring af engraftment af humane inducerede…
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Biochemistry

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JoVE Journal Biochemistry
Forbedring af engraftment af humane inducerede pluripotente stamcelle afledte Kardiomyocytter via en forbigående hæmning af Rho Kinaseaktivitet
 
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Forbedring af engraftment af humane inducerede pluripotente stamcelle afledte Kardiomyocytter via en forbigående hæmning af Rho Kinaseaktivitet

Article DOI: 10.3791/59452-v • 08:00 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1,2, 1, 1,3, 1, 1, 1, 2, 1, 1
1Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham, 2Division of Cardiovascular Diseases, School of Medicine, School of Engineering, University of Alabama at Birmingham, 3Department of Cardiac Surgery, The Second Xiangya Hospital of Central South University

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I denne protokol viser og uddyber vi, hvordan man bruger menneskeskabte pluripotente stamceller til differentiering og rensning af kardiomyocytter, og yderligere om, hvordan man kan forbedre sin transplantations effektivitet med Rho-associeret proteinkinasehæmmer forbehandling i en mus myokardieinfarkt model.

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Biokemi pluripotente stamceller kardiomyocytter celleterapi myokardieinfarkt Rho kinase klippe hæmmer
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