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1Oxford Centre for Diabetes, Endocrinology and Metabolism,University of Oxford, 2Lund University Diabetes Centre, Unit of Molecular Metabolism, Clinical Research Centre,Malmö University HospitalMalmö, 3Institute of Neuroscience of Physiology, Department of Physiology, Metabolic Research Unit,University of Göteborg, 4Oxford National Institute for Health Research,Biomedical Research Centre, 5Life and Medical Sciences,University of Hertfordshire
Chapters
- 00:04Title
- 00:30Calcium (Ca2+) Imaging Dynamics
- 02:21Microscope Setup
- 03:48Time-Lapse Imaging
- 04:45Numerical Data Analysis
- 06:16Results: Representative Islet Cell Responses to Various Stimuli
- 07:26Conclusion
Here, we present a protocol for imaging and quantifying calcium dynamics in heterogeneous cell populations, such as pancreatic islet cells. Fluorescent reporters are delivered into the peripheral layer of cells within the islet, which is then immobilized and imaged, and per-cell analysis of the dynamics of fluorescence intensity is performed.
