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A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii
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Chapters
- 00:04Title
- 00:32Chlorophyll, Lipid, and Metabolite Extraction and Fraction Allocation
- 02:04Polar (Primary) and Non-Polar (Lipid) Metabolite and Chlorophyll Content Determination
- 03:35Extraction and Determination of Protein Content, Digestion, and Analysis
- 05:53Results: Representative Synchronized Culture Analyses
- 07:21Conclusion
System-wide analysis of multiple biomolecules is crucial to gain functional and mechanistic insights into biological processes. Hereby, an extensive protocol is described for high throughput extraction of lipids, metabolites, proteins and starch from a single sample harvested from synchronized Chlamydomonas culture.
