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活鼠中多萨尔希波坎卡尔CA1的纵向双光成像

JoVE Journal
Behavior

This content is Free Access.

JoVE Journal Behavior

Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice

Please note that all translations are automatically generated. Click here for the English version.

活鼠中多萨尔希波坎卡尔CA1的纵向双光成像

DOI:

10.3791/59598-v

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09:34 min

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June 19, 2019

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Alessandro F. Ulivi, Tim P. Castello-Waldow, Ghabiba Weston², Long Yan, Ryohei Yasuda, Alon Chen⁴, Alessio Attardo

¹Dept. of Stress Neurobiology and Neurogenetics,Max Planck Institute of Psychiatry, ²Graduate School of Systemic Neurosciences,Ludwig Maximilians University, ³Max Planck Florida Institute for Neuroscience, ⁴Dept. of Neurobiology,Weizmann Institute of Science

Chapters

00:04Title
01:04Preparation of the Imaging Cannula
02:11Implantation of the Imaging Cannula Over the Dorsal Hippocampus
05:32Imaging Preparation
06:16Cannula Imaging
07:10Results: Longitudinal Imaging of Dendritic Structure and Dendritic Spines Dynamics
08:50Conclusion

Summary

Automatic Translation

这种方法描述了一种慢性制剂,允许光学访问活小鼠的海马区。这种制剂可用于在几周内对神经元结构可塑性和活动诱发细胞可塑性进行纵向光学成像。

Tags

Keywords: Two-photon Imaging Dorsal Hippocampus CA1 Live Mice Longitudinal Memory Formation Memory Recall Alzheimer’s Disease Survival Surgery Imaging Cannula UV Curing Dental Drill Lambda Bregma Lidocaine Periosteum

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