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Journal
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Biochemistry
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无核酸酶的表达和纯化原氧基质 3,4-二氧酶
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Biochemistry
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JoVE Journal
Biochemistry
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
Please note that all translations are automatically generated.
Click here for the English version.
无核酸酶的表达和纯化原氧基质 3,4-二氧酶
DOI:
10.3791/59599-v
•
10:14 min
•
November 08, 2019
•
Ryan K. Messer
,
Miguel A. Lopez Jr.
,
Gayan Senavirathne
,
Kristine E. Yoder
1
Cancer Biology and Genetics, College of Medicine
,
The Ohio State University
Chapters
00:04
Title
00:25
Inducing PCD (protocatechuate 3,4 dioxygenase) Expression in
E. coli
03:39
Nickel Affinity Chromatography Purification of PCD
04:46
Nuclease Activity Assay
06:19
PCA (Protocatechuic Acid) Oxidation and Nuclease Activity Assays
08:01
Results: Purification of PCD
09:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
原酸3,4-二氧酶(PCD)可以酶分从水系统中分离游离的游离双原子氧,使用其基质原酸(PCA)。该协议描述了这种氧气清除酶的表达、纯化和活性分析。
Tags
Nuclease-free
Oxygen Scavenger
Protocatechuate 3
4-dioxygenase
PCD Expression
E. Coli BL21
Transformation
LB-ampicillin Agar
Protein Expression
IPTG
Ammonium Iron Sulfate
Centrifugation
SDS-PAGE
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