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Uttryck och rening av Nukleasfri syre avskiljare Protocatechuat 3, 4-dioxygenas
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
DOI:

10:14 min

November 08, 2019

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Chapters

  • 00:04Title
  • 00:25Inducing PCD (protocatechuate 3,4 dioxygenase) Expression in E. coli
  • 03:39Nickel Affinity Chromatography Purification of PCD
  • 04:46Nuclease Activity Assay
  • 06:19PCA (Protocatechuic Acid) Oxidation and Nuclease Activity Assays
  • 08:01Results: Purification of PCD
  • 09:23Conclusion

Summary

Automatic Translation

Protocatechuat 3, 4-dioxygenase (PCD) kan enzymatiskt avlägsna fritt diatomärt syre från ett vattensystem med hjälp av dess substrat protocatechuic Acid (PCA). Detta protokoll beskriver uttrycket, renings-och aktivitets analysen av detta enzym för syre rensning.

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