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Chapters
- 00:04Title
- 00:59Mouse Smooth Muscle Actin (SMA) Cell Labeling and Lacrimal Gland (LG) Harvesting and Microdissection
- 02:10LG Single-cell Suspension Preparation
- 04:46Antibody Staining and Fluorescence-activated Cell Sorting (FACS)
- 05:45Results: Representative MEC and Pericyte Characterization and Isolation
- 06:38Conclusion
Lakrimal bezi (LG), α-pürüzsüz Kas aksini (αSMA) ifade eden iki hücre türüne sahiptir: miyoepitelyal hücreler (MECs) ve perikayt. MECS ektodermal orijinli, birçok glandüler dokularda bulunan, perikayt endodermal kökeni vasküler pürüzsüz Kas hücreleri iken. Bu protokol, murine LGs 'den MECs ve perikaytları yalıtır.
