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Mise en œuvre d'un microscope non linéaire basé sur la diffusion stimulée de Raman
JoVE Journal
Engineering
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JoVE Journal Engineering
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
DOI:

09:13 min

July 06, 2019

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Chapters

  • 00:04Title
  • 00:58Alignment of the Microscope Spatial Beam Overlap
  • 01:58Alignment of the Microscope Temporal Synchronization of the Beams Use of the Autocorrelator
  • 03:24Alignment of the Microscope Aligning the Femtosecond Laser Beams :OPO and Ti:Sa
  • 04:44Optimization of Temporal Synchronization of Beams
  • 05:48Optimization of Spatial Overlap of Beams – Transmission Image Acquisition of OPO and Ti:Sa
  • 06:48SRS Image Acquisition
  • 07:40Results: Nonlinear Microscope Based on Stimulated Raman Scattering
  • 08:43Conclusion

Summary

Automatic Translation

Dans ce manuscrit, la mise en œuvre d'un microscope à diffusion Raman (SRS) stimulé, obtenue par l'intégration d'un sER expérimental avec un microscope à balayage laser, est décrite. Le microscope SRS est basé sur deux sources laser femtoseconde (fs), un Ti-Sapphire (Ti:Sa) et un oscillateur paramétrique optique synchronisé (OPO).

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