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Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

DOI:
10.3791/59617-v

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08:09 min

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June 19, 2019

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, , , , , , ,
1Division of Inflammation Biology,La Jolla Institute for Immunology, 2Flow Cytometry Core Facility,La Jolla Institute for Immunology

Chapters

  • 00:04Title
  • 01:41Bone Marrow (BM) Harvest
  • 03:40BM Cell Staining and Mass Cytometry (CyTOF) Analysis Preparation
  • 06:15Results: Representative Whole Bone Marrow CyTOF Analysis of Neutrophil-lineage Cells
  • 07:47Conclusion

Summary

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Automatic Translation

Here, we present a protocol to process fresh bone marrow (BM) isolated from mouse or human for high-dimensional mass cytometry (Cytometry by Time-Of-Flight, CyTOF) analysis of neutrophil-lineage cells.

Tags

Mass CytometryBone MarrowNeutrophil-lineage CellsMyeloid CellsCell PreservationC57 Black Six MouseTibiaFemurDissection

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