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使用 Ever 设计自动化、高通量、连续细胞生长实验
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
DOI:

07:26 min

May 19, 2019

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Chapters

  • 00:04Title
  • 00:58Sterilizing the System
  • 02:56Loading the Culture Vials
  • 03:51Configuring eVOLVER Software and Programming Algorithmic Culture Routines
  • 04:48Inoculating and Monitoring Experiment in Real-time
  • 05:47Results: Fitness Surface Construction
  • 06:35Conclusion

Summary

Automatic Translation

EVOLVER 框架可实现高通量连续微生物培养, 具有高分辨率和对实验参数的动态控制。该协议演示了如何应用该系统进行复杂的健身实验, 指导用户对许多文化进行自动化控制编程, 实时测量、收集和与实验数据交互。

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