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用于口腔中性粒细胞病评价的鼠期牙周炎的强结合诱导模型
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
DOI:

07:15 min

January 21, 2020

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Chapters

  • 00:04Title
  • 01:15Ligature Installation
  • 03:29Sample Collection
  • 05:05Antibody Staining for Flow Cytometric Analysis
  • 06:03Results: FACS and Alveolar Bone Loss Analyses
  • 06:37Conclusion

Summary

Automatic Translation

本文提出了建立涉及多个上颌骨的月牙病结扎诱导模型的协议,从而在涉及牙龈组织和骨骼的较大区域进行后续分析,并减少动物使用。还介绍了一种以类似于人类受试者的方式评估口服中性粒细胞的技术。

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